Journal: Cancers

Article Title: Unraveling the Role of Guanylate-Binding Proteins (GBPs) in Breast Cancer: A Comprehensive Literature Review and New Data on Prognosis in Breast Cancer Subtypes

doi: 10.3390/cancers14112794

Figure Lengend Snippet: Manuscripts addressing GBP-1 in breast cancer.

Article Snippet: SAS, HepG2, KB, MM102 cells , - , Cells made clinically cells radioresistant (CRR). All CRR cells expressed elevated GBP compared to parental cells. KD of GBP reduced radioresistance. , Fukumoto, 2014 [ ] .

Techniques: Expressing, Activation Assay, In Vitro, In Vivo, Incubation

Journal: Pharmaceuticals

Article Title: Pharmacological Properties of Trichostatin A, Focusing on the Anticancer Potential: A Comprehensive Review

doi: 10.3390/ph15101235

Figure Lengend Snippet: Direct anticancer activity of trichostatin A.

Article Snippet: Purchased , Human oral SCC cell line SAS, Ca9-22, and HSC , MTT assay Flow cytometry analysis Western blot analysis RT-PCR Confocal laser microscopic analysis , Enhanced the replication of the HSV-1 mutant through the activation of NF-κB Inhibited cell growth by inducing cell cycle arrest at G 1 , [ ] .

Techniques: Activity Assay, Immunofluorescence, Immunoprecipitation, Western Blot, MTT Assay, Cell Cycle Assay, Staining, Membrane, Activation Assay, Flow Cytometry, Proliferation Assay, De-Phosphorylation Assay, Inhibition, Soft Agar Assay, Confocal Microscopy, Quantitative RT-PCR, Expressing, Gene Expression, Nucleic Acid Electrophoresis, Extraction, Colony Assay, TUNEL Assay, Chromatin Immunoprecipitation, In Vivo, Formulation, DNA Fragmentation Assay, RNA Extraction, Reporter Assay, Transmission Assay, Electron Microscopy, MTT Viability Assay, Real-time Polymerase Chain Reaction, Phospho-proteomics, Blocking Assay, Immunocytochemistry, Isolation, Northern Blot, Cell Migration Assay, Migration, Caspase Activity Assay, Immunopeptidomics, BrdU Staining, Transwell Migration Assay, Virus, Luciferase, Mutagenesis, Transgenic Assay, Electric Cell-substrate Impedance Sensing, Fluorescence, Microscopy, MSP Assay, Transfection, Hybridization, Wound Healing Assay, Lactate Dehydrogenase Assay, Caspase-3 Activity Assay, Agarose Gel Electrophoresis, ATP Assay, Light Microscopy, Derivative Assay, Microarray, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Cell Culture, In Vitro, Apoptosis Assay, Tube Formation Assay, Rnase Protection Assay, CCK-8 Assay, Permeability, Histopathology, Transformation Assay, Clinical Proteomics, Release Assay, Produced, Immunohistochemical staining, Injection, Invasion Assay, Over Expression, Concentration Assay, Ubiquitin Proteomics, Binding Assay, Immunostaining, Quantitative Proteomics, Caspase Assay, Fourier Transform Infrared Spectroscopy, Spectroscopy, Clonogenic Assay

Journal: Pharmaceuticals

Article Title: Pharmacological Properties of Trichostatin A, Focusing on the Anticancer Potential: A Comprehensive Review

doi: 10.3390/ph15101235

Figure Lengend Snippet: Anticancer activity of TSA through sensitization.

Article Snippet: Purchased , Human oral SCC cell line SAS, Ca9-22, and HSC , MTT assay Flow cytometry analysis Western blot analysis RT-PCR Confocal laser microscopic analysis , Enhanced the replication of the HSV-1 mutant through the activation of NF-κB Inhibited cell growth by inducing cell cycle arrest at G 1 , [ ] .

Techniques: Activity Assay, MTT Assay, Annexin V Assay, DNA Extraction, Immunofluorescence, Western Blot, Proliferation Assay, Staining, Flow Cytometry, Expressing, Wound Healing Assay, Immunocytochemistry, Isolation, Alamar Blue Assay, Membrane, Clonogenic Cell Survival Assay, Clonogenic Assay, Immunohistochemistry, Inhibition, CCK-8 Assay, In Vivo, Activation Assay, RNA Extraction, Immunoprecipitation, Mmp Assay, Electrophoresis, Mass Spectrometry, Microarray, In Vitro, Caspase Activity Assay, Cell Cycle Assay, TUNEL Assay, Injection, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay

Journal: Biochemistry and Biophysics Reports

Article Title: miR-30a attenuates drug sensitivity to 5-FU by modulating cell proliferation possibly by downregulating cyclin E2 in oral squamous cell carcinoma

doi: 10.1016/j.bbrep.2021.101114

Figure Lengend Snippet: Cytotoxic effects of 5-FU against 5-FU-sensitive and -resistant OSCC cells and miR-30a overexpression in resistant cells. (A and B) Cell survival of parental (SAS, Ca9-22) and resistant (SAS/FR2, Ca9-22/FR2) cell lines was monitored 72 h after incubation with various concentrations of 5-FU using a cell proliferation assay. Results represent means ± SD of three independent experiments. (C) Total RNA was extracted and miR-30a expression was analyzed using RT-qPCR. The results represent the mean ± SD of three independent experiments. * P < 0.05.

Article Snippet: * P < 0.05. (C) Cell survival was monitored in control (SAS/st-cont, Ca9-22/st-cont) and miR-30a-over-expressing (SAS/st-30a, Ca9-22/st-30a) cell lines 72 h after incubation with various concentrations of 5-FU.

Techniques: Over Expression, Incubation, Proliferation Assay, Expressing, Quantitative RT-PCR

Journal: Biochemistry and Biophysics Reports

Article Title: miR-30a attenuates drug sensitivity to 5-FU by modulating cell proliferation possibly by downregulating cyclin E2 in oral squamous cell carcinoma

doi: 10.1016/j.bbrep.2021.101114

Figure Lengend Snippet: Overexpression of miR-30a affects cell cycle distribution and 5-FU sensitivity in OSCC cells. (A) Expression of miR-30a in stable miR-30a-overexpressing cells (SAS/st-30a and Ca9-22/st-30a) and control cells (SAS/st-cont and Ca9-22/st-cont). Total RNA was extracted and miR-30a expression was analyzed using RT-qPCR. (B) For cell cycle analysis, the PI-stained DNA content of the cells was evaluated using FACS cytometry. Results represent means ± SD of three independent experiments. * P < 0.05. (C) Cell survival was monitored in control (SAS/st-cont, Ca9-22/st-cont) and miR-30a-over-expressing (SAS/st-30a, Ca9-22/st-30a) cell lines 72 h after incubation with various concentrations of 5-FU. Results represent means ± SD of three independent experiments. (D) Apoptotic cells were detected using Annexin V-APC after 72 h with 2.0 μg/ml 5-FU treatment. Results represent means ± SD of three independent experiments. * P < 0.05; ** P < 0.01.

Article Snippet: * P < 0.05. (C) Cell survival was monitored in control (SAS/st-cont, Ca9-22/st-cont) and miR-30a-over-expressing (SAS/st-30a, Ca9-22/st-30a) cell lines 72 h after incubation with various concentrations of 5-FU.

Techniques: Over Expression, Expressing, Control, Quantitative RT-PCR, Cell Cycle Assay, Staining, Cytometry, Incubation

Journal: Biochemistry and Biophysics Reports

Article Title: miR-30a attenuates drug sensitivity to 5-FU by modulating cell proliferation possibly by downregulating cyclin E2 in oral squamous cell carcinoma

doi: 10.1016/j.bbrep.2021.101114

Figure Lengend Snippet: Effect of miR-30a knockdown on proliferation, cell cycle, and 5-FU sensitivity in miR-30a-overexpressing cells. (A) Si-miR-30a was transfected into miR-30a-overexpressing cells (SAS/st-30a, Ca9-22/st-30a) using the 24-well plates (1 × 10 4 cells per well). After 48 h, total RNA was isolated and evaluated using RT-qPCR. (B) Cell proliferation without 5-FU treatment was determined 72 h after transfection. (C) PI-stained DNA content of the cells was evaluated using FACS cytometry 48 h after transfection. (D) After further 72 h after 1 μg/ml 5-FU treatment following 48 h transfection, cell survival was monitored. Results represent the means ± SD of three independent experiments. * P < 0.05; ** P < 0.01.

Article Snippet: * P < 0.05. (C) Cell survival was monitored in control (SAS/st-cont, Ca9-22/st-cont) and miR-30a-over-expressing (SAS/st-30a, Ca9-22/st-30a) cell lines 72 h after incubation with various concentrations of 5-FU.

Techniques: Knockdown, Transfection, Isolation, Quantitative RT-PCR, Staining, Cytometry

Journal: Journal of Nutrition and Metabolism

Article Title: Exploring the Role of Licorice and Its Derivatives in Cell Signaling Pathway NF- κ B and MAPK

doi: 10.1155/2024/9988167

Figure Lengend Snippet: Effect of licorice on MAPK pathway.

Article Snippet: 11. , Oral squamous cell carcinoma (OSCC) generally known as oral cancer caused by tobacco and alcohol , Glycyrrhiza glabra and Glycyrrhiza uralensis , Semilicoisoflavone B (SFB) , Human OSCC cell lines SAS, HSC3M3, OECM-1, and SCC9 , Reduction of MAPK along with Ras/Raf/MEK signaling , [ ] .

Techniques: Activation Assay, Infection, Virus, Phospho-proteomics, Expressing, Inhibition